Gabriele‘s paper entitled ‘Multiplexed protein maps link subcellular organization to cellular states’ has been accepted for publication. Congratulations!!
This work reports the development of iterative indirect immunofluoresence imaging (4i), which allows 40-plex immunostaining of hundreds of thousands of single cells using conventional primary and secondary antibodies in high-throughput. Combined with cellular computer vision, this generates a wealth of quantitative information about biological samples from the millimeter to the nanometer scale. It also reports a novel pixel-based computational approach that allows the unsupervised comprehensive quantification of protein sub-compartmentalization within various multicellular, cellular, and pharmacological contexts. These approaches have enormous potential for biomedicine in both fundamental research, diagnostics and personalized medicine.