High throughput screening microscope

IMG_0729As the flagship of screening microscopes, this fully automated high-throughput screening microscope can image in confocal spinning disc as well as in epi-fluorescent mode. With it’s three 14×16.6mm Andor sCMOS camera’s (4 times the usual EMCCD chip surface) and the newest model of wide-field CSU-W1 microlense enhanced dual Nipkow disc that has been designed to fully illuminate the extended camera chip, it can capture a full well of a 384 plate in just 4 images at 10x magnification.

The CV7K is able to read 6,24,96,384 and 1536 wells plates and to perform live cell imaging over multiple days with (almost) no cytotoxicity in a temperature, humidity and CO2 controlled environment.

The system will soon be equipped with an automated plate loader, so it can run autonomously for long periods of time. This microscope is used for many projects that involve the acquisition of images of very large numbers of cells at very high resolution, as well as for high-throughput image-based screens.

 

Specifications:

  • Objectives: 10x NA 0.4 and 20x NA0.75 air plus 40x NA 1.15 and 60x NA 1.2 water immersion with automated water supply
  • 3 Andor sCMOS camera’s, 2560×2160 pixels, 14×16.6mm wide-view area
  • 4 solid state lasers, 405, 488, 561, 640 nm, power of respectively 100,200,200,100 mW
  • Confocal and epi-fluorescent observation mode
  • Stage incubator, lense temperature control, from Room temperature to 40 degrees.
  • Software controlled CO2 mixer and stage humidifier